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61.
用不同剂量的60Co-γ射线诱变钝顶螺旋藻Spirulinaplatensis出发株(Sp)IS-90010,筛选获得两株抗高光抑制突变株(Sp)AIp-90010和(Sp)AIp-90011,然后比较出发株和突变株的一般形态和生理生化特性。出发株和突变株的一般形态有较大的差异,与出发株相比,两个突变株藻丝体显著变短,螺旋数目大大减小。出发株是对高光敏感的品系,而突变株表现明显的抗高光抑制,13000lx光强下出发株和两个突变株的代时分别为29.4、20.8和22.2h。(Sp)AIp-90011的光合作用和呼吸作用与出发株相似,而(Sp)AIp-90010明显地表现出高光合、低呼吸作用。突变株和出发株都属于中温品系,最适温度为28℃,具有较广的温度适应范围(23~35℃)以及相同的耐盐性,但突变株的生长速率比出发株快、代时短。此外三者的蛋白质含量、氨基酸组成差别不大:(Sp)AIp-90011的可溶性多糖比出发株减少40%。而在(Sp)AIp-90010中其含量提高60%。 相似文献
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64.
核糖核酸(RNA)与稀土铽离子在pH5.0~6.5条件下能形成具有较强荧光的络合物,其激发峰在288nm处,发射峰为494nm及545nm处.RNA在0.1~10mg/L范围内与其荧光强度呈线性关系,其检出限为6.0×10 ̄(-8)mol/L.腺苷酸,尿苷酸,胞苷酸对RNA的干扰比较小,因此可在其存在下选择性地测定RNA. 相似文献
65.
从皖南尖吻蝮蛇(Agkistrodonacutus)毒液中经DEAE-Sepharose和SephacrylS-200两步凝胶柱层析首次纯化出一种中分子量出血毒素(简称AaHⅣ).经SDS-PAGE和等电聚焦凝胶电泳测定其分子量为44kD,等电点为pH5.0.从500mg粗毒中可获得20mgAaHⅣ纯品.AaHⅣ有较强的出血活性,最小出血剂量(MHD)为0.4μg. 相似文献
66.
Cell signaling and heat shock protein expression 总被引:5,自引:0,他引:5
Exposure of cells and organs to heat shock is associated with numerous changes in various cellular metabolic parameters and overexpression of proteins collectively known as heat shock proteins (HSP). In this communication we review the cell-signaling events that are altered in response to heat shock as they relate to the subsequent induction of HSP 70 kd (HSP-70) expression. We also review the mechanisms by which HSP-70 is involved in conferring cytoprotective effects. The possibility of altering HSP expression through manipulations of the cell-signal process has clinical importance.The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of the Army or Department of Defense. 相似文献
67.
Phosphatase Activity Toward Abnormally Phosphorylated τ: Decrease in Alzheimer Disease Brain 总被引:1,自引:0,他引:1
Cheng-Xin Gong Sadia Shaikh Jian-Zhi Wang Tanweer Zaidi Inge Grundke-Iqbal Khalid Iqbal 《Journal of neurochemistry》1995,65(2):732-738
Abstract: Microtubule-associated protein τ is abnormally hyperphosphorylated and aggregated in affected neurons of Alzheimer disease brain. This hyperphosphorylated τ can be dephosphorylated at some of the abnormal phosphorylated sites by purified protein phosphatase-1, 2A, and 2B in vitro. In the present study, we have developed an assay to measure protein phosphatase activity toward τ-1 sites (Ser199 /Ser202 ) using the hyperphosphorylated τ isolated from Alzheimer disease brain as substrate. Using this assay, we have identified that in normal brain, protein phosphatase-2A and 2B and, to a lesser extent, 1 are involved in the dephosphorylation of τ. The K m values of dephosphorylation of the hyperphosphorylated τ by protein phosphatase-2A and 2B are similar. The τ phosphatase activity is decreased by ∼30% in brain of Alzheimer disease patients compared with those of age-matched controls. These findings suggest that a defect of protein phosphatase could be the cause of the abnormal hyperphosphorylation of τ in Alzheimer disease. 相似文献
68.
The role of context on alpha-helix stabilization: host-guest analysis in a mixed background peptide model. 总被引:1,自引:1,他引:0 下载免费PDF全文
J. Yang E. J. Spek Y. Gong H. Zhou N. R. Kallenbach 《Protein science : a publication of the Protein Society》1997,6(6):1264-1272
The helix content of a series of peptides containing single substitutions of the 20 natural amino acids in a new designed host sequence, succinyl-YSEEEEKAKKAXAEEAEKKKK-NH2, has been determined using CD spectroscopy. This host is related to one previously studied, in which triple amino acid substitutions were introduced into a background of Glu-Lys blocks completely lacking alanine. The resulting free energies show that only Ala and Glu- prove to be helix stabilizing, while all other side chains are neutral or destabilizing. This agrees with results from studies of alanine-rich peptide modela, but not the previous Glu-Lys block oligomers in which Leu and Met also stabilize helix. The helix propensity scale derived from the previous block oligomers correlated well with the frequencies of occurrence of different side chains in helical sequences of proteins, whereas the values from the present series do not. The role of context in determining scales of helix propensity values is discussed, and the ability of algorithms designed to predict helix structure from sequence is compared. 相似文献
69.
昆虫卵黄发生研究进展 总被引:19,自引:4,他引:15
昆虫卵黄发生研究进展李乾君,龚和,管致和(中国科学院动物研究所北京100080)(北京农业大学植保系北京100094)昆虫卵的成熟一般分为三个时期--卵黄发生前期(Previtellogenicstage)、卵黄发生期(vitellogenicsta... 相似文献
70.
Structural analysis of the N- and C-termini in a peptide with consensus sequence. 总被引:3,自引:2,他引:1 下载免费PDF全文
Y. Gong H. X. Zhou M. Guo N. R. Kallenbach 《Protein science : a publication of the Protein Society》1995,4(8):1446-1456
We present a structural analysis of a peptide, the sequence of which includes amino acids that show preferences for specific positions near the N- and C-termini in protein helices. This peptide has the sequence ac-YMSEDELKAAEAAFKRHGVP-amide, which includes a strong version of an N-terminal Harper-Rose capping box structure as well as a Gly located close to the C-terminus designed to elucidate its role in C-terminal capping. The sequence of five residues at the middle is inserted to separate effects at the two ends via a helix-stabilizing linker. Application of a simulated annealing procedure using interproton distance constraints derived from 1H NOESY experiments in water reveals the presence of a C-terminal structure in this model. The C-terminus forms a folded back structure in a significant fraction of structures generated by the annealing, in most of which Gly assumes an alpha L conformation. This structure occurs within a highly flexible region of the molecule and hence is occupied only a fraction of the time. 相似文献